Step 1 of 4
How many samples
are you pooling?
are you pooling?
Enter the number of samples. Each sample will need a barcode, amplicon length, and concentration in the next step.
samples
Supports 2 to 24 samples per pool.
Step 2 of 4
Sample details
Enter the barcode, amplicon length (bp), and measured concentration (ng/µL) for each sample after barcoding PCR.
#Sample nameBarcodeLength (bp)Conc. (ng/µL)
Step 3 of 4
Barcoding volumes
Volumes to add per sample for normalisation before barcoding. Total volume per sample is 8 µL.
| # | Sample | Barcode | Conc. (ng/µL) | Sample vol. (µL) | Water vol. (µL) | Conc. after (ng/µL) |
|---|
Step 4 of 4
Pooling volumes
Volumes to take from each barcoded sample for equimolar pooling. Based on the lowest molarity in the pool.
| # | Sample | Conc. after (ng/µL) | Mol. weight (g/mol) | Molarity (nM) | Volume for pool (µL) |
|---|